YwkE

• Description: similar to N5-glutamine methyltransferase that modifies peptide release factors
  
  

Gene name	ywkE
Synonyms
Essential	no
Product	N5-glutamine methyltransferase
Function	peptide release factor methylation
MW, pI	32 kDa, 4.89
Gene length, protein length	864 bp, 288 aa
Immediate neighbours	ywkF, prfA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation, protein modification

The gene

Basic information

• Locus tag: BSU37000

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: hemK family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1NV8 (the protein from Thermotoga maritima) PubMed

• UniProt: P45873

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ywkE PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Heidi L Schubert, John D Phillips, Christopher P Hill
Structures along the catalytic pathway of PrmC/HemK, an N5-glutamine AdoMet-dependent methyltransferase.
Biochemistry: 2003, 42(19);5592-9
[PubMed:12741815] [WorldCat.org] [DOI] (P p)

Valérie Heurgué-Hamard, Stéphanie Champ, Ake Engström, Måns Ehrenberg, Richard H Buckingham
The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors.
EMBO J: 2002, 21(4);769-78
[PubMed:11847124] [WorldCat.org] [DOI] (P p)

Kenji Nakahigashi, Naoko Kubo, Shin-ichiro Narita, Takeshi Shimaoka, Simon Goto, Taku Oshima, Hirotada Mori, Maki Maeda, Chieko Wada, Hachiro Inokuchi
HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination.
Proc Natl Acad Sci U S A: 2002, 99(3);1473-8
[PubMed:11805295] [WorldCat.org] [DOI] (P p)