MetE

• Description: methionine synthase
  
  

Gene name	metE
Synonyms	metC
Essential	no
Product	methionine synthase
Function	biosynthesis of methionine
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	86 kDa, 4.839
Gene length, protein length	2286 bp, 762 aa
Immediate neighbours	guaD, ispA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, phosphoproteins

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU13180

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine (according to Swiss-Prot)

• Protein family: vitamin-B12 independent methionine synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on ser/ thr/ tyr PubMed, S-cysteinylation after diamide stress (C719) PubMed
  • S-bacillithiolation upon hypochlorite stress on Cys-730 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1T7L (from Thermotoga maritima, 44% identity, 61% similarity) PubMed

• UniProt: P80877

• KEGG entry: [2]

• E.C. number: 2.1.1.14

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: metE

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed
  • repressed by methionine (about 1000-fold)(S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: