YidC2

• Description: Sec-independent membrane protein translocase
  
  

Gene name	yqjG
Synonyms
Essential	no
Product	Sec-independent membrane protein translocase
Function	membrane insertion of proteins and protein secretion
MW, pI	30 kDa, 9.903
Gene length, protein length	825 bp, 275 aa
Immediate neighbours	mifM, yqjF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein secretion, membrane proteins

This gene is a member of the following regulons

MifM regulon

The gene

Basic information

• Locus tag: BSU23890

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: Type 2 subfamily (according to Swiss-Prot)

• Paralogous protein(s): SpoIIIJ, one of the two proteins has to be present for viability PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P54544

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yqjG (according to DBTBS)

• Sigma factor:

• Regulation:
  • repressed by large amounts of SpoIIIJ, induced by small amounts of SpoIIIJ (MifM) PubMed

• Regulatory mechanism:
  • MifM: "attenuation mechanism", an mRNA hairpin of the yqjG transcript unfold upon translation arrest of MifM at decreased SpoIIIJ levels PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Manfred J Saller, Andreas Otto, Greetje A Berrelkamp-Lahpor, Dörte Becher, Michael Hecker, Arnold J M Driessen
Bacillus subtilis YqjG is required for genetic competence development.
Proteomics: 2011, 11(2);270-82
[PubMed:21204254] [WorldCat.org] [DOI] (I p)

Shinobu Chiba, Anne Lamsa, Kit Pogliano
A ribosome-nascent chain sensor of membrane protein biogenesis in Bacillus subtilis.
EMBO J: 2009, 28(22);3461-75
[PubMed:19779460] [WorldCat.org] [DOI] (I p)

Manfred J Saller, Fabrizia Fusetti, Arnold J M Driessen
Bacillus subtilis SpoIIIJ and YqjG function in membrane protein biogenesis.
J Bacteriol: 2009, 191(21);6749-57
[PubMed:19717609] [WorldCat.org] [DOI] (I p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)

Aileen Rubio, Xin Jiang, Kit Pogliano
Localization of translocation complex components in Bacillus subtilis: enrichment of the signal recognition particle receptor at early sporulation septa.
J Bacteriol: 2005, 187(14);5000-2
[PubMed:15995216] [WorldCat.org] [DOI] (P p)

Harold Tjalsma, Sierd Bron, Jan Maarten van Dijl
Complementary impact of paralogous Oxa1-like proteins of Bacillus subtilis on post-translocational stages in protein secretion.
J Biol Chem: 2003, 278(18);15622-32
[PubMed:12586834] [WorldCat.org] [DOI] (P p)

Takako Murakami, Koki Haga, Michio Takeuchi, Tsutomu Sato
Analysis of the Bacillus subtilis spoIIIJ gene and its Paralogue gene, yqjG.
J Bacteriol: 2002, 184(7);1998-2004
[PubMed:11889108] [WorldCat.org] [DOI] (P p)