MrpD

• Description: Na+/H+ antiporter subunit, multiple resistance and pH homeostasis
  
  

Gene name	mrpD
Synonyms	yufD
Essential	yes PubMed
Product	Na+/H+ antiporter subunit
Function	sodium export
MW, pI	53 kDa, 9.488
Gene length, protein length	1479 bp, 493 aa
Immediate neighbours	mrpC, mrpE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), essential genes, membrane proteins

The gene

Basic information

• Locus tag: BSU31630

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: MrpA-MrpB-MrpC-MrpD-MrpE-MrpF-MrpG (complex)

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O05229

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mrpA-mrpB-mrpC-mrpD-mrpE-mrpF-mrpG PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yusuke Kajiyama, Masato Otagiri, Junichi Sekiguchi, Toshiaki Kudo, Saori Kosono
The MrpA, MrpB and MrpD subunits of the Mrp antiporter complex in Bacillus subtilis contain membrane-embedded and essential acidic residues.
Microbiology (Reading): 2009, 155(Pt 7);2137-2147
[PubMed:19389778] [WorldCat.org] [DOI] (P p)

Yusuke Kajiyama, Masato Otagiri, Junichi Sekiguchi, Saori Kosono, Toshiaki Kudo
Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
J Bacteriol: 2007, 189(20);7511-4
[PubMed:17693497] [WorldCat.org] [DOI] (P p)

M Ito, A A Guffanti, W Wang, T A Krulwich
Effects of nonpolar mutations in each of the seven Bacillus subtilis mrp genes suggest complex interactions among the gene products in support of Na(+) and alkali but not cholate resistance.
J Bacteriol: 2000, 182(20);5663-70
[PubMed:11004162] [WorldCat.org] [DOI] (P p)

M Ito, A A Guffanti, B Oudega, T A Krulwich
mrp, a multigene, multifunctional locus in Bacillus subtilis with roles in resistance to cholate and to Na+ and in pH homeostasis.
J Bacteriol: 1999, 181(8);2394-402
[PubMed:10198001] [WorldCat.org] [DOI] (P p)