LmrA

• Description: transcriptional regulator, control of yxaG-yxaH and lmrA-lmrB
  
  

Gene name	lmrA
Synonyms	lin-2, yccB
Essential	no
Product	transcriptional regulator
Function	regulation of lincomycin resistance
MW, pI	20 kDa, 5.033
Gene length, protein length	564 bp, 188 aa
Immediate neighbours	lmrB, ansZ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription factors and their control, resistance against toxins/ antibiotics

This gene is a member of the following regulons

LmrA regulon

The gene

Basic information

• Locus tag: BSU02680

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): YxaF

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: flavonoids such as quercetin serve as inducers, binding results in release from DNA PubMed

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34619

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lmrA-lmrB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by flavonoids such as quercetin (LmrA)PubMed

• Regulatory mechanism:
  • LmrA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kazutake Hirooka, Satoshi Kunikane, Hiroshi Matsuoka, Ken-Ichi Yoshida, Kanako Kumamoto, Shigeo Tojo, Yasutaro Fujita
Dual regulation of the Bacillus subtilis regulon comprising the lmrAB and yxaGH operons and yxaF gene by two transcriptional repressors, LmrA and YxaF, in response to flavonoids.
J Bacteriol: 2007, 189(14);5170-82
[PubMed:17483215] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Yo-Hei Ohki, Makiko Murata, Masaki Kinehara, Hiroshi Matsuoka, Takenori Satomura, Reiko Ohki, Miyuki Kumano, Kunio Yamane, Yasutaro Fujita
Bacillus subtilis LmrA is a repressor of the lmrAB and yxaGH operons: identification of its binding site and functional analysis of lmrB and yxaGH.
J Bacteriol: 2004, 186(17);5640-8
[PubMed:15317768] [WorldCat.org] [DOI] (P p)

Miyuki Kumano, Masaya Fujita, Kouji Nakamura, Makiko Murata, Reiko Ohki, Kunio Yamane
Lincomycin resistance mutations in two regions immediately downstream of the -10 region of lmr promoter cause overexpression of a putative multidrug efflux pump in Bacillus subtilis mutants.
Antimicrob Agents Chemother: 2003, 47(1);432-5
[PubMed:12499232] [WorldCat.org] [DOI] (P p)