ComX

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  • Description: competence pheromone precursor, transfers cell density signal to ComP; triggers the production of surfactin

Gene name comX
Synonyms
Essential no
Product competence pheromone precursor
Function quorum sensing
MW, pI 6 kDa, 4.358
Gene length, protein length 165 bp, 55 aa
Immediate neighbours comP, comQ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ComX context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

genetic competence, quorum sensing

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU31700

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: is synthesized as an inactive precursor, cleaved, and modified (isoprenylated) by ComQ prior to excretion
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: secreted (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

The ComX --> surfactin signaling pathway is an example for paracrine signaling in bacteria

References

Daniel López, Hera Vlamakis, Richard Losick, Roberto Kolter
Paracrine signaling in a bacterium.
Genes Dev: 2009, 23(14);1631-8
[PubMed:19605685] [WorldCat.org] [DOI] (I p)

Kassem Hamze, Daria Julkowska, Sabine Autret, Krzysztof Hinc, Krzysztofa Nagorska, Agnieszka Sekowska, I Barry Holland, Simone J Séror
Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC.
Microbiology (Reading): 2009, 155(Pt 2);398-412
[PubMed:19202088] [WorldCat.org] [DOI] (P p)

Masahiro Okada, Hisao Yamaguchi, Isao Sato, Soo Jeong Cho, David Dubnau, Youji Sakagami
Structure-activity relationship studies on quorum sensing ComX(RO-E-2) pheromone.
Bioorg Med Chem Lett: 2007, 17(6);1705-7
[PubMed:17240141] [WorldCat.org] [DOI] (P p)

Masahiro Okada, Isao Sato, Soo Jeong Cho, Hidehisa Iwata, Toshihiko Nishio, David Dubnau, Youji Sakagami
Structure of the Bacillus subtilis quorum-sensing peptide pheromone ComX.
Nat Chem Biol: 2005, 1(1);23-4
[PubMed:16407988] [WorldCat.org] [DOI] (P p)

Masahiro Okada, Isao Sato, Soo Jeong Cho, Yoshihiro Suzuki, Makoto Ojika, David Dubnau, Youji Sakagami
Towards structural determination of the ComX pheromone: synthetic studies on peptides containing geranyltryptophan.
Biosci Biotechnol Biochem: 2004, 68(11);2374-87
[PubMed:15564679] [WorldCat.org] [DOI] (P p)

M Ansaldi, D Dubnau
Diversifying selection at the Bacillus quorum-sensing locus and determinants of modification specificity during synthesis of the ComX pheromone.
J Bacteriol: 2004, 186(1);15-21
[PubMed:14679219] [WorldCat.org] [DOI] (P p)

Mireille Ansaldi, Darja Marolt, Tina Stebe, Ines Mandic-Mulec, David Dubnau
Specific activation of the Bacillus quorum-sensing systems by isoprenylated pheromone variants.
Mol Microbiol: 2002, 44(6);1561-73
[PubMed:12067344] [WorldCat.org] [DOI] (P p)

P Tortosa, L Logsdon, B Kraigher, Y Itoh, I Mandic-Mulec, D Dubnau
Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system.
J Bacteriol: 2001, 183(2);451-60
[PubMed:11133937] [WorldCat.org] [DOI] (P p)

Y Weinrauch, T Msadek, F Kunst, D Dubnau
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis.
J Bacteriol: 1991, 173(18);5685-93
[PubMed:1715859] [WorldCat.org] [DOI] (P p)