NasF

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  • Description: uroporphyrinogen methyltransferase

Gene name nasF
Synonyms nasBE
Essential no
Product uroporphyrinogen methyltransferase
Function nitrate respiration
MW, pI 53 kDa, 6.523
Gene length, protein length 1449 bp, 483 aa
Immediate neighbours ycgT, nasE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NasF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU03280

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

respiration, utilization of nitrogen sources other than amino acids

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1 (according to Swiss-Prot)
  • Protein family: precorrin methyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • expressed under anaerobic conditions (ResD) PubMed
    • induced by nitric oxide under anaerobic conditions (NsrR) PubMed
    • induced by iron starvation (Fur) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References