NfrA

• Description: Spx-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein
  
  

Gene name	nfrA
Synonyms	ywcG, ipa-43d
Essential	no
Product	FMN-containing NADPH-linked nitro/flavin reductase
Function	unknown
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	28 kDa, 5.732
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	ywcH, rodA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38110

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD(+) degrading activity leading to free nicotinamide PubMed

• Protein family: flavin oxidoreductase frp family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3N2S PubMed

• UniProt: P39605

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nfrA-ywcH PubMed

• Sigma factor: SigA PubMed, SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • Spx: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sylvie Cortial, Philippe Chaignon, Bogdan I Iorga, Stéphane Aymerich, Gilles Truan, Virginie Gueguen-Chaignon, Philippe Meyer, Solange Moréra, Jamal Ouazzani
NADH oxidase activity of Bacillus subtilis nitroreductase NfrA1: insight into its biological role.
FEBS Lett: 2010, 584(18);3916-22
[PubMed:20727352] [WorldCat.org] [DOI] (I p)

Le Thi Tam, Haike Antelmann, Christine Eymann, Dirk Albrecht, Jörg Bernhardt, Michael Hecker
Proteome signatures for stress and starvation in Bacillus subtilis as revealed by a 2-D gel image color coding approach.
Proteomics: 2006, 6(16);4565-85
[PubMed:16847875] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Shunji Nakano, Michiko M Nakano, Ying Zhang, Montira Leelakriangsak, Peter Zuber
A regulatory protein that interferes with activator-stimulated transcription in bacteria.
Proc Natl Acad Sci U S A: 2003, 100(7);4233-8
[PubMed:12642660] [WorldCat.org] [DOI] (P p)

C Moch, O Schrögel, R Allmansberger
Transcription of the nfrA-ywcH operon from Bacillus subtilis is specifically induced in response to heat.
J Bacteriol: 2000, 182(16);4384-93
[PubMed:10913069] [WorldCat.org] [DOI] (P p)

C Moch, O Schrögel, R Allmansberger
The sigmaD-dependent transcription of the ywcG gene from Bacillus subtilis is dependent on an excess of glucose and glutamate.
Mol Microbiol: 1998, 27(5);889-98
[PubMed:9535080] [WorldCat.org] [DOI] (P p)