Rph

• Description: RNase PH, 3'-5' exoribonuclease
  
  

Gene name	rph
Synonyms
Essential	no
Product	RNase PH (EC 2.7.7.56)
Function	3'-5' exoribonuclease
MW, pI	26 kDa, 4.888
Gene length, protein length	735 bp, 245 aa
Immediate neighbours	ysnA, gerM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28370

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

Rnases, translation

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • major player in the exonucleolytic maturation of CCA-containing tRNA precursors PubMed
  • responsible for endonucleolytic cleavage of scRNA (scr) PubMed

• Protein family: RNase PH family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1OYS

• UniProt: P28619

• KEGG entry: [2]

• E.C. number: 2.7.7.56

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage