Fbp

• Description: fructose-1,6-bisphosphatase, involved in gluconeogenesis
  
  

Gene name	fbp
Synonyms	fdp, yydE
Essential	no
Product	fructose-1,6-bisphosphatase
Function	gluconeogenesis
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism
MW, pI	77 kDa, 5.601
Gene length, protein length	2013 bp, 671 aa
Immediate neighbours	yydF, yydD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU40190

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate + H₂O = D-fructose 6-phosphate + phosphate (according to Swiss-Prot)

• Protein family: FBPase class 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3D1R (from E. coli, 49% identity, 68% similarity)PubMed

• UniProt: Q45597

• KEGG entry: [3]

• E.C. number: 3.1.3.11

Expression and regulation

• Operon: fpb PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Greg Brown, Alexander Singer, Vladimir V Lunin, Michael Proudfoot, Tatiana Skarina, Robert Flick, Samvel Kochinyan, Ruslan Sanishvili, Andrzej Joachimiak, Aled M Edwards, Alexei Savchenko, Alexander F Yakunin
Structural and biochemical characterization of the type II fructose-1,6-bisphosphatase GlpX from Escherichia coli.
J Biol Chem: 2009, 284(6);3784-92
[PubMed:19073594] [WorldCat.org] [DOI] (P p)

Y Fujita, K Yoshida, Y Miwa, N Yanai, E Nagakawa, Y Kasahara
Identification and expression of the Bacillus subtilis fructose-1, 6-bisphosphatase gene (fbp).
J Bacteriol: 1998, 180(16);4309-13
[PubMed:9696785] [WorldCat.org] [DOI] (P p)

Y Fujita, E Freese
Isolation and properties of a Bacillus subtilis mutant unable to produce fructose-bisphosphatase.
J Bacteriol: 1981, 145(2);760-7
[PubMed:6257649] [WorldCat.org] [DOI] (P p)

Y Fujita, E Freese
Purification and properties of fructose-1,6-bisphosphatase of Bacillus subtilis.
J Biol Chem: 1979, 254(12);5340-9
[PubMed:221467] [WorldCat.org] (P p)