OxdD

• Description: oxalate decarboxylase, spore coat protein
  
  

Gene name	oxdD
Synonyms	yoaN
Essential	no
Product	oxalate decarboxylase
Function	unknown
MW, pI	43 kDa, 5.359
Gene length, protein length	1176 bp, 392 aa
Immediate neighbours	yoaM, yoaO
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU18670

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

sporulation proteins, resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Oxalate = formate + CO₂ (according to Swiss-Prot)

• Protein family: UPF0361 family (according to Swiss-Prot)

• Paralogous protein(s): OxdC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34767

• KEGG entry: [3]

• E.C. number: 4.1.1.2

Expression and regulation

• Operon: yoaN PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Miia R Mäkelä, Kristiina Hildén, Taina K Lundell
Oxalate decarboxylase: biotechnological update and prevalence of the enzyme in filamentous fungi.
Appl Microbiol Biotechnol: 2010, 87(3);801-14
[PubMed:20464388] [WorldCat.org] [DOI] (I p)

Original publications

Sébastien Potot, Cláudia R Serra, Adriano O Henriques, Ghislain Schyns
Display of recombinant proteins on Bacillus subtilis spores, using a coat-associated enzyme as the carrier.
Appl Environ Microbiol: 2010, 76(17);5926-33
[PubMed:20601499] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Teresa Costa, Leif Steil, Lígia O Martins, Uwe Völker, Adriano O Henriques
Assembly of an oxalate decarboxylase produced under sigmaK control into the Bacillus subtilis spore coat.
J Bacteriol: 2004, 186(5);1462-74
[PubMed:14973022] [WorldCat.org] [DOI] (P p)

A Tanner, L Bowater, S A Fairhurst, S Bornemann
Oxalate decarboxylase requires manganese and dioxygen for activity. Overexpression and characterization of Bacillus subtilis YvrK and YoaN.
J Biol Chem: 2001, 276(47);43627-34
[PubMed:11546787] [WorldCat.org] [DOI] (P p)