XynA

• Description: endo-1,4-beta-xylanase
  
  

Gene name	xynA
Synonyms
Essential	no
Product	endo-1,4-beta-xylanase
Function	xylan degradation
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	23 kDa, 9.644
Gene length, protein length	639 bp, 213 aa
Immediate neighbours	pps, yobD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU18840

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure: 2QZ3 (complex with xylotetraose), 2Z79, 3EXU

• UniProt: P18429

• KEGG entry: [3]

• E.C. number: 3.2.1.8

Expression and regulation

• Operon: xynA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Louise E Rasmussen, Jens F Sørensen, Anne S Meyer
Kinetics and substrate selectivity of a Triticum aestivum xylanase inhibitor (TAXI) resistant D11F/R122D variant of Bacillus subtilis XynA xylanase.
J Biotechnol: 2010, 146(4);207-14
[PubMed:20188130] [WorldCat.org] [DOI] (I p)

Annick Pollet, Stijn Lagaert, Elena Eneyskaya, Anna Kulminskaya, Jan A Delcour, Christophe M Courtin
Mutagenesis and subsite mapping underpin the importance for substrate specificity of the aglycon subsites of glycoside hydrolase family 11 xylanases.
Biochim Biophys Acta: 2010, 1804(4);977-85
[PubMed:20096384] [WorldCat.org] [DOI] (P p)

Louise E Rasmussen, Anne S Meyer
Size exclusion chromatography for the quantitative profiling of the enzyme-catalyzed hydrolysis of Xylo-oligosaccharides.
J Agric Food Chem: 2010, 58(2);762-9
[PubMed:19994888] [WorldCat.org] [DOI] (I p)

Tim Beliën, Iris J Joye, Jan A Delcour, Christophe M Courtin
Computational design-based molecular engineering of the glycosyl hydrolase family 11 B. subtilis XynA endoxylanase improves its acid stability.
Protein Eng Des Sel: 2009, 22(10);587-96
[PubMed:19531602] [WorldCat.org] [DOI] (I p)

Annick Pollet, Elien Vandermarliere, Jeroen Lammertyn, Sergei V Strelkov, Jan A Delcour, Christophe M Courtin
Crystallographic and activity-based evidence for thumb flexibility and its relevance in glycoside hydrolase family 11 xylanases.
Proteins: 2009, 77(2);395-403
[PubMed:19422059] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

C Lindner, J Stülke, M Hecker
Regulation of xylanolytic enzymes in Bacillus subtilis.
Microbiology (Reading): 1994, 140 ( Pt 4);753-7
[PubMed:8012596] [WorldCat.org] [DOI] (P p)