UvrA

• Description: excinuclease ABC (subunit A)
  
  

Gene name	uvrA
Synonyms
Essential	no
Product	excinuclease ABC (subunit A)
Function	DNA repair after UV damage
MW, pI	105 kDa, 5.843
Gene length, protein length	2871 bp, 957 aa
Immediate neighbours	yvzB, uvrB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35160

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UvrA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Mfd-UvrA PubMed, UvrA-UvrB PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2R6F (Geobacillus stearothermophilus)

• UniProt: O34863

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: uvrB-uvrA (according to DBTBS)

• Sigma factor:

• Regulation:
  • induced upon DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• mutant: GP1175 (uvrAB::ermC) (available in the Stülke lab)

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

James J Truglio, Deborah L Croteau, Bennett Van Houten, Caroline Kisker
Prokaryotic nucleotide excision repair: the UvrABC system.
Chem Rev: 2006, 106(2);233-52
[PubMed:16464004] [WorldCat.org] [DOI] (P p)

Bennett Van Houten, Deborah L Croteau, Matthew J DellaVecchia, Hong Wang, Caroline Kisker
'Close-fitting sleeves': DNA damage recognition by the UvrABC nuclease system.
Mutat Res: 2005, 577(1-2);92-117
[PubMed:15927210] [WorldCat.org] [DOI] (P p)

A Sancar
Mechanisms of DNA excision repair.
Science: 1994, 266(5193);1954-6
[PubMed:7801120] [WorldCat.org] [DOI] (P p)

Original Publications

Bradley T Smith, Alan D Grossman, Graham C Walker
Localization of UvrA and effect of DNA damage on the chromosome of Bacillus subtilis.
J Bacteriol: 2002, 184(2);488-93
[PubMed:11751826] [WorldCat.org] [DOI] (P p)

C M Lovett, K C Cho, T M O'Gara
Purification of an SOS repressor from Bacillus subtilis.
J Bacteriol: 1993, 175(21);6842-9
[PubMed:8226626] [WorldCat.org] [DOI] (P p)