AhpC

• Description: alkyl hydroperoxide reductase (small subunit)
  
  

Gene name	ahpC
Synonyms	perR
Essential	no
Product	alkyl hydroperoxide reductase (small subunit)
Function	resistance against peroxide stres
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	20 kDa, 4.283
Gene length, protein length	561 bp, 187 aa
Immediate neighbours	gntZ, ahpF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU40090

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

resistance against oxidative and electrophile stress, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 R'-SH + ROOH = R'-S-S-R' + H₂O + ROH (according to Swiss-Prot)

• Protein family: ahpC/TSA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1WE0 (from Amphibacillus xylanus, 78% identity, 88% similarity) PubMed

• UniProt: P80239

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ahpC-ahpF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by H2O2 (PerR) PubMed

• Regulatory mechanism:
  • PerR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alain Lévine, Françoise Vannier, Cédric Absalon, Lauriane Kuhn, Peter Jackson, Elaine Scrivener, Valérie Labas, Joëlle Vinh, Patrick Courtney, Jérôme Garin, Simone J Séror
Analysis of the dynamic Bacillus subtilis Ser/Thr/Tyr phosphoproteome implicated in a wide variety of cellular processes.
Proteomics: 2006, 6(7);2157-73
[PubMed:16493705] [WorldCat.org] [DOI] (P p)

A F Herbig, J D Helmann
Roles of metal ions and hydrogen peroxide in modulating the interaction of the Bacillus subtilis PerR peroxide regulon repressor with operator DNA.
Mol Microbiol: 2001, 41(4);849-59
[PubMed:11532148] [WorldCat.org] [DOI] (P p)

N Bsat, L Chen, J D Helmann
Mutation of the Bacillus subtilis alkyl hydroperoxide reductase (ahpCF) operon reveals compensatory interactions among hydrogen peroxide stress genes.
J Bacteriol: 1996, 178(22);6579-86
[PubMed:8932315] [WorldCat.org] [DOI] (P p)

H Antelmann, S Engelmann, R Schmid, M Hecker
General and oxidative stress responses in Bacillus subtilis: cloning, expression, and mutation of the alkyl hydroperoxide reductase operon.
J Bacteriol: 1996, 178(22);6571-8
[PubMed:8932314] [WorldCat.org] [DOI] (P p)