AlsS

From SubtiWiki
Revision as of 14:51, 30 November 2010 by Lflorez (talk | contribs)
Jump to: navigation, search
  • Description: acetolactate synthase

Gene name alsS
Synonyms
Essential no
Product acetolactate synthase
Function overflow metabolism
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 61 kDa, 5.164
Gene length, protein length 1713 bp, 571 aa
Immediate neighbours alsD, alsR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AlsS context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU36010

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

carbon core metabolism

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO2 (according to Swiss-Prot)
  • Protein family: TPP enzyme family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induction by acetate (AlsR) PubMed
    • repressed as long as terminal electron acceptors are available for respiration (Rex) PubMed
    • subject to positive stringent control upon lysine starvation PubMed

Note: since acetate formation requires ackA activation by CcpA there is an indirect effect of CcpA on the alsSD operon: the operon is not expressed in ccpA mutants

  • Regulatory mechanism:
    • stringent response: due to presence of adenines at +1 and +2 positions of the transcript PubMed
    • AlsR: transcription activation in the presence of acetate PubMed
    • Rex: transcription repression if the ratio NADH2/NAD is high PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References