LmrA

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Gene name lmrA
Synonyms lin-2, yccB
Essential no
Product transcriptional regulator
Function regulation of lincomycin resistance
MW, pI 20 kDa, 5.033
Gene length, protein length 564 bp, 188 aa
Immediate neighbours lmrB, ansZ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LmrA context.gif
This image was kindly provided by SubtiList




The gene

Basic information

  • Locus tag: BSU02680

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): YxaF

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: flavonoids such as quercetin serve as inducers, binding results in release from DNA PubMed
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by flavonoids such as quercetin (LmrA)PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kazutake Hirooka, Satoshi Kunikane, Hiroshi Matsuoka, Ken-Ichi Yoshida, Kanako Kumamoto, Shigeo Tojo, Yasutaro Fujita
Dual regulation of the Bacillus subtilis regulon comprising the lmrAB and yxaGH operons and yxaF gene by two transcriptional repressors, LmrA and YxaF, in response to flavonoids.
J Bacteriol: 2007, 189(14);5170-82
[PubMed:17483215] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Yo-Hei Ohki, Makiko Murata, Masaki Kinehara, Hiroshi Matsuoka, Takenori Satomura, Reiko Ohki, Miyuki Kumano, Kunio Yamane, Yasutaro Fujita
Bacillus subtilis LmrA is a repressor of the lmrAB and yxaGH operons: identification of its binding site and functional analysis of lmrB and yxaGH.
J Bacteriol: 2004, 186(17);5640-8
[PubMed:15317768] [WorldCat.org] [DOI] (P p)

Miyuki Kumano, Masaya Fujita, Kouji Nakamura, Makiko Murata, Reiko Ohki, Kunio Yamane
Lincomycin resistance mutations in two regions immediately downstream of the -10 region of lmr promoter cause overexpression of a putative multidrug efflux pump in Bacillus subtilis mutants.
Antimicrob Agents Chemother: 2003, 47(1);432-5
[PubMed:12499232] [WorldCat.org] [DOI] (P p)