Des

• Description: phospholipid desaturase
  
  

Gene name	des
Synonyms	yocE
Essential	no
Product	phospholipid desaturase
Function	adaptation of membrane fluidity at low temperatures
MW, pI	40 kDa, 9.88
Gene length, protein length	1056 bp, 352 aa
Immediate neighbours	yocD, desK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU19180

Phenotypes of a mutant

cold-sensitive PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34653

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: des PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced upon cold shock (DesR) PubMed
  • induced at optimal growth temperature (37°C) (DesR) PubMed
  • repressed at increased membrane fluidity PubMed

• Regulatory mechanism:
  • two component system DesR-DesK: transcription activation PubMed, PubMed
  • two component system DesR-DesK in presence of anteiso-branched fatty acid primers: transcription inhibition PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Diego de Mendoza, Universidad Nacional de Rosario, Argentine homepage