YocH

• Description: peptidoglycan hydrolase (amidase)
  
  

Gene name	yocH
Synonyms
Essential	no
Product	peptidoglycan hydrolase
Function	cell wall turnover
MW, pI	30 kDa, 8.371
Gene length, protein length	861 bp, 287 aa
Immediate neighbours	desR, yocI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU19210

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: O34669

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yocH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during logrithmic growth (AbrB) PubMed
  • induced in response to cell wall derived muropeptides derived from growing cells but not lysed cells (requires activities of PrkC and YocH) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: AH024 knock-out mutant (kan) available in the lab of Kevin Devine

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Kevin Devine