RnjA

• Description: RNase J1
  
  

Gene name	rnjA
Synonyms	ykqC
Essential	yes PubMed
Product	RNase J1
Function	RNA processing
Metabolic function and regulation of this protein in SubtiPathways: Ile, Leu, Val, Coenzyme A
MW, pI	61 kDa, 5.902
Gene length, protein length	1665 bp, 555 aa
Immediate neighbours	adeC, ykzG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU14530

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: endonuclease and 5'-3' exonuclease

• Protein family: RNase J subfamily (according to Swiss-Prot)

• Paralogous protein(s): RnjB

RNAs affected by rnjA

hbs, thrS

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: RnjA-RnjB PubMed, RnjA-PfkA PubMed, RnjA-PnpA PubMed, RnjA-Rny PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3BK1 (RNase J from Thermus thermophilus) 3BK2 (RNase J from Thermus thermophilus, complex with UMP)

• UniProt: Q45493

• KEGG entry: [2]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

required for thrS RNA processing, involved in maturation of the 5’-end of the16S rRNA

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed, subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant: GP41 (rnjA under control of p(xyl)), available in Stülke lab; SSB342 (rnjA under pspac), cat, available in Harald Putzer lab

• Expression vector:

• lacZ fusion: pGP418 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• FLAG-tag construct: GP1020 (spc, based on pGP1331), available in the Stülke lab

• Antibody:

Labs working on this gene/protein

Harald Putzer, IBPC Paris, France Homepage

David Bechhofer, Mount Sinai School, New York, USA Homepage

Ciaran Condon, IBPC, Paris, France Homepage