YaaJ

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  • Description: tRNA specific adenosine deaminase

Gene name yaaJ
Synonyms
Essential no
Product tRNA specific adenosine deaminase
Function tRNA modification
MW, pI 17 kDa, 5.636
Gene length, protein length 483 bp, 161 aa
Immediate neighbours yaaI, scr
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YaaJ context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU00180

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: cytidine and deoxycytidylate deaminase family (according to Swiss-Prot)
  • Paralogous protein(s):
  • The corresponding gene in E. coli: EcoliWiki

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 2B3J (the protein of Staphylococcus aureus, complex with RNA) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Takashi Inaoka, Takenori Satomura, Yasutaro Fujita, Kozo Ochi
Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
FEMS Microbiol Lett: 2009, 291(2);151-6
[PubMed:19087206] [WorldCat.org] [DOI] (I p)

Heather C Losey, Alexander J Ruthenburg, Gregory L Verdine
Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA.
Nat Struct Mol Biol: 2006, 13(2);153-9
[PubMed:16415880] [WorldCat.org] [DOI] (P p)

Jeannette Wolf, André P Gerber, Walter Keller
tadA, an essential tRNA-specific adenosine deaminase from Escherichia coli.
EMBO J: 2002, 21(14);3841-51
[PubMed:12110595] [WorldCat.org] [DOI] (P p)