MsrA

• Description: peptide methionine sulfoxide reductase
  
  

Gene name	msrA
Synonyms	yppP
Essential	no
Product	peptide methionine sulfoxide reductase
Function	regeneration of methionine and restoration of protein function after oxidative damage
MW, pI	20 kDa, 5.709
Gene length, protein length	531 bp, 177 aa
Immediate neighbours	msrB, ypoP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU21690

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

resistance against oxidative and electrophile stress

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Peptide-L-methionine + thioredoxin disulfide + H₂O = peptide-L-methionine (S)-S-oxide + thioredoxin (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54154

• KEGG entry: [2]

• E.C. number: 1.8.4.11

Expression and regulation

• Operon: msrA-msrB PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by oxidative stress (Spx)

• Regulatory mechanism: Spx: oxidized Spx binds RpoA and enhances msrA-msrB operon transcription PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

CongHui You, Agnieszka Sekowska, Olivera Francetic, Isabelle Martin-Verstraete, YiPing Wang, Antoine Danchin
Spx mediates oxidative stress regulation of the methionine sulfoxide reductases operon in Bacillus subtilis.
BMC Microbiol: 2008, 8;128
[PubMed:18662407] [WorldCat.org] [DOI] (I e)