Hmp

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  • Description: flavohemoglobin, involved in resistance to nitric oxide (NO)

Gene name hmp
Synonyms ykiA
Essential no
Product flavohemoglobin
Function resistance to NO
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 44 kDa, 5.705
Gene length, protein length 1197 bp, 399 aa
Immediate neighbours ykhA, ykzH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Hmp context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU13040

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 NO + 2 O2 + NAD(P)H = 2 NO3- + NAD(P)+ (according to Swiss-Prot)
  • Protein family: FAD-binding FR-type domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under anaerobic conditions (ResD) PubMed
    • repressed unless NO is present under anaerobic conditions (NsrR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Falko Hochgräfe, Carmen Wolf, Stephan Fuchs, Manuel Liebeke, Michael Lalk, Susanne Engelmann, Michael Hecker
Nitric oxide stress induces different responses but mediates comparable protein thiol protection in Bacillus subtilis and Staphylococcus aureus.
J Bacteriol: 2008, 190(14);4997-5008
[PubMed:18487332] [WorldCat.org] [DOI] (I p)

Michiko M Nakano, Hao Geng, Shunji Nakano, Kazuo Kobayashi
The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression.
J Bacteriol: 2006, 188(16);5878-87
[PubMed:16885456] [WorldCat.org] [DOI] (P p)

Charles M Moore, Michiko M Nakano, Tao Wang, Rick W Ye, John D Helmann
Response of Bacillus subtilis to nitric oxide and the nitrosating agent sodium nitroprusside.
J Bacteriol: 2004, 186(14);4655-64
[PubMed:15231799] [WorldCat.org] [DOI] (P p)

M M Nakano, Y Zhu, M Lacelle, X Zhang, F M Hulett
Interaction of ResD with regulatory regions of anaerobically induced genes in Bacillus subtilis.
Mol Microbiol: 2000, 37(5);1198-207
[PubMed:10972836] [WorldCat.org] [DOI] (P p)

M LaCelle, M Kumano, K Kurita, K Yamane, P Zuber, M M Nakano
Oxygen-controlled regulation of the flavohemoglobin gene in Bacillus subtilis.
J Bacteriol: 1996, 178(13);3803-8
[PubMed:8682784] [WorldCat.org] [DOI] (P p)