YyaF

• Description: GTP-binding protein/ GTPase
  
  

Gene name	yyaF
Synonyms
Essential	no
Product	GTP-binding protein/ GTPase
Function	unknown
MW, pI	39 kDa, 4.659
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	rpsF, yyaE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU40920

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP

• Protein family: EngD subfamily (according to Swiss-Prot) Era/Obg family

• Paralogous protein(s): Era, Obg, YphC, YsxC, RbgA, YqeH, ThdF, YyaF, YnbA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P37518

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP836, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP844, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

References

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)