BglH

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  • Description: phospho-beta-glucosidase

Gene name bglH
Synonyms
Essential no
Product phospho-beta-glucosidase
Function salicin utilization
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 53 kDa, 4.957
Gene length, protein length 1407 bp, 469 aa
Immediate neighbours yxiE, bglP
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BglH context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU39260

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 1 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
    • CcpA: transcription repression, (CcpA binding site overlaps -35 region) PubMed
    • LicT-dependent RNA switch (antitermination, lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HPr PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Juliane Ollinger, Kyung-Bok Song, Haike Antelmann, Michael Hecker, John D Helmann
Role of the Fur regulon in iron transport in Bacillus subtilis.
J Bacteriol: 2006, 188(10);3664-73
[PubMed:16672620] [WorldCat.org] [DOI] (P p)

B Setlow, R-M Cabrera-Martinez, P Setlow
Mechanism of the hydrolysis of 4-methylumbelliferyl-beta-D-glucoside by germinating and outgrowing spores of Bacillus species.
J Appl Microbiol: 2004, 96(6);1245-55
[PubMed:15139916] [WorldCat.org] [DOI] (P p)

Barbara Setlow, Arturo Cabrera-Hernandez, Rosa Martha Cabrera-Martinez, Peter Setlow
Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis.
Arch Microbiol: 2004, 181(1);60-7
[PubMed:14652714] [WorldCat.org] [DOI] (P p)

S Krüger, M Hecker
Regulation of the putative bglPH operon for aryl-beta-glucoside utilization in Bacillus subtilis.
J Bacteriol: 1995, 177(19);5590-7
[PubMed:7559347] [WorldCat.org] [DOI] (P p)

D Le Coq, C Lindner, S Krüger, M Steinmetz, J Stülke
New beta-glucoside (bgl) genes in Bacillus subtilis: the bglP gene product has both transport and regulatory functions similar to those of BglF, its Escherichia coli homolog.
J Bacteriol: 1995, 177(6);1527-35
[PubMed:7883710] [WorldCat.org] [DOI] (P p)