TcyP

• Description: cystine transporter, membrane protein
  
  

Gene name	tcyP
Synonyms	yhcL
Essential	no
Product	cystine transporter, membrane protein
Function	cystine uptake
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	48 kDa, 9.471
Gene length, protein length	1389 bp, 463 aa
Immediate neighbours	yhcK, yhcM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU09130

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: cystine-proton symporter PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: K(m) = 0.6 myM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane associated PubMed

Database entries

• Structure:

• UniProt: P54596

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: tcyP

• Sigma factor:

• Regulation: repressed in the presence of cysteine (CymR) PubMed
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism: CymR: transcription repression PubMed
  • CymR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Sergine Even, Pierre Burguière, Sandrine Auger, Olga Soutourina, Antoine Danchin, Isabelle Martin-Verstraete
Global control of cysteine metabolism by CymR in Bacillus subtilis.
J Bacteriol: 2006, 188(6);2184-97
[PubMed:16513748] [WorldCat.org] [DOI] (P p)

Pierre Burguière, Sandrine Auger, Marie-Françoise Hullo, Antoine Danchin, Isabelle Martin-Verstraete
Three different systems participate in L-cystine uptake in Bacillus subtilis.
J Bacteriol: 2004, 186(15);4875-84
[PubMed:15262924] [WorldCat.org] [DOI] (P p)

Sandrine Auger, Antoine Danchin, Isabelle Martin-Verstraete
Global expression profile of Bacillus subtilis grown in the presence of sulfate or methionine.
J Bacteriol: 2002, 184(18);5179-86
[PubMed:12193636] [WorldCat.org] [DOI] (P p)