IlvB

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  • Description: acetolactate synthase (large subunit)

Gene name ilvB
Synonyms
Essential no
Product acetolactate synthase (large subunit)
Function biosynthesis of branched-chain amino acids
Metabolic function and regulation of this protein in SubtiPathways:
Ile, Leu, Val, Coenzyme A
MW, pI 62 kDa, 5.178
Gene length, protein length 1722 bp, 574 aa
Immediate neighbours ilvH, ysnD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
IlvB context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU28310

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO2 (according to Swiss-Prot)
  • Protein family: TPP enzyme family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1N53 (T box RNA)
  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation: repressed by casamino acids PubMed, repressed by CodY PubMed, expressed in the absence of branched-chain amino acids (BCAA), expression is stimulated in the presence of glucose (CcpA) PubMed, during growth repressed under nitrogen limiting conditions (TnrA) PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism: glucose regulation: CcpA PubMed, TnrA: transcription repession PubMed, repression by BCAA: tRNA-controlled RNA switch (T-box) that mediates termination/antitermination, CodY: transcription repression PubMed1 PubMed2
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant: GP324, ilvB under control of pXyl (cat), available in Jörg Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References