FtsH

• Description: ATP-dependent metalloprotease
  
  

Gene name	ftsH
Synonyms
Essential	no
Product	ATP-dependent metalloprotease
Function	cell division, sporulation initiation
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	70 kDa, 5.841
Gene length, protein length	1911 bp, 637 aa
Immediate neighbours	hprT, coaX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00690

Phenotypes of a mutant

strongly reduced sporulation frequency

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: degrades Spo0E, resulting in reduced sporulation frequency in a ftsH mutant

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), membrane PubMed

Database entries

• Structure:

• UniProt: P37476

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ftsH PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by heat shock (class III)

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ai Thi Thuy Le, Wolfgang Schumann
The Spo0E phosphatase of Bacillus subtilis is a substrate of the FtsH metalloprotease.
Microbiology (Reading): 2009, 155(Pt 4);1122-1132
[PubMed:19332814] [WorldCat.org] [DOI] (P p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Matthias Kotschwar, Evita Harfst, Tanja Ohanjan, Wolfgang Schumann
Construction and analyses of mutant ftsH alleles of Bacillus subtilis involving the ATPase- and Zn-binding domains.
Curr Microbiol: 2004, 49(3);180-5
[PubMed:15386101] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

Stephan Zellmeier, Ulrich Zuber, Wolfgang Schumann, Thomas Wiegert
The absence of FtsH metalloprotease activity causes overexpression of the sigmaW-controlled pbpE gene, resulting in filamentous growth of Bacillus subtilis.
J Bacteriol: 2003, 185(3);973-82
[PubMed:12533473] [WorldCat.org] [DOI] (P p)

R S Prajapati, T Ogura, S M Cutting
Structural and functional studies on an FtsH inhibitor from Bacillus subtilis.
Biochim Biophys Acta: 2000, 1475(3);353-9
[PubMed:10913836] [WorldCat.org] [DOI] (P p)

W Wehrl, M Niederweis, W Schumann
The FtsH protein accumulates at the septum of Bacillus subtilis during cell division and sporulation.
J Bacteriol: 2000, 182(13);3870-3
[PubMed:10851010] [WorldCat.org] [DOI] (P p)

W Schumann
FtsH--a single-chain charonin?
FEMS Microbiol Rev: 1999, 23(1);1-11
[PubMed:10077851] [WorldCat.org] [DOI] (P p)

M M Nakano, Y P Dailly, P Zuber, D P Clark
Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth.
J Bacteriol: 1997, 179(21);6749-55
[PubMed:9352926] [WorldCat.org] [DOI] (P p)

Elena Lysenko, Teru Ogura, Simon M Cutting
Characterization of the ftsH gene of Bacillus subtilis.
Microbiology (Reading): 1997, 143 ( Pt 3);971-978
[PubMed:9084181] [WorldCat.org] [DOI] (P p)

E Deuerling, A Mogk, C Richter, M Purucker, W Schumann
The ftsH gene of Bacillus subtilis is involved in major cellular processes such as sporulation, stress adaptation and secretion.
Mol Microbiol: 1997, 23(5);921-33
[PubMed:9076729] [WorldCat.org] [DOI] (P p)

E Deuerling, B Paeslack, W Schumann
The ftsH gene of Bacillus subtilis is transiently induced after osmotic and temperature upshift.
J Bacteriol: 1995, 177(14);4105-12
[PubMed:7608085] [WorldCat.org] [DOI] (P p)