UreB

• Description: urease (beta subunit)
  
  

Gene name	ureB
Synonyms
Essential	no
Product	urease (beta subunit)
Function	utilization of urea as alternative nitrogen source
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	13 kDa, 9.751
Gene length, protein length	372 bp, 124 aa
Immediate neighbours	ureC, ureA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU36650

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Urea + H₂O = CO₂ + 2 NH₃ (according to Swiss-Prot)

• Protein family: urease beta subunit family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P71035

• KEGG entry: [3]

• E.C. number: 3.5.1.5

Expression and regulation

• Operon: ureA-ureB-ureC PubMed

• Sigma factor: SigA, SigH PubMed

• Regulation: induced by nitrogen limitation (GlnR, TnrA) PubMed, repressed by CodY PubMed
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism: GlnR: transcription repression PubMed, TnrA: transcription activation PubMed, CodY: transcription repression PubMed
  • TnrA: transcription activation PubMed
  • [PucR]]: transcription activation PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: