Difference between revisions of "MetK"

From SubtiWiki
Jump to: navigation, search
Line 13: Line 13:
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || S-adenosylmethionine synthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || S-adenosylmethionine synthesis
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 5.025   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 5.025   

Revision as of 15:21, 16 June 2009

  • Description: S-adenosylmethionine synthetase

Gene name metK
Synonyms metE
Essential yes PubMed
Product S-adenosylmethionine synthetase
Function S-adenosylmethionine synthesis
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 43 kDa, 5.025
Gene length, protein length 1200 bp, 400 aa
Immediate neighbours asnB, pckA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MetK context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU30550

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine (according to Swiss-Prot)
  • Protein family: AdoMet synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation: induced by methionine starvation (S-box) PubMed
  • Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References