Difference between revisions of "NrdE"

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* '''Regulation:''' distinct promoters for aerobic and anaerobic expression [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed], anaerobic expression requires [[ResD]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
 
* '''Regulation:''' distinct promoters for aerobic and anaerobic expression [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed], anaerobic expression requires [[ResD]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
 +
** expressed under anaerobic conditions ([[ResD]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
  
 
* '''Regulatory mechanism:''' [[ResD]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
 
* '''Regulatory mechanism:''' [[ResD]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
 +
** [[ResD]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/16885274 PubMed]
  
 
* '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
 
* '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]

Revision as of 17:14, 11 June 2009

  • Description: ribonucleoside-diphosphate reductase (major subunit)

Gene name nrdE
Synonyms
Essential yes PubMed
Product ribonucleoside-diphosphate reductase (major subunit)
Function synthesis of deoxyribonucleoside triphosphates
MW, pI 80 kDa, 5.931
Gene length, protein length 2100 bp, 700 aa
Immediate neighbours nrdI, nrdF
Gene sequence (+200bp) Protein sequence
Genetic context
NrdE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU17380

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
  • Protein family: ribonucleoside diphosphate reductase large chain family (according to Swiss-Prot)
  • Paralogous protein(s): YosN

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation: distinct promoters for aerobic and anaerobic expression PubMed, anaerobic expression requires ResD PubMed
    • expressed under anaerobic conditions (ResD) PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Elisabeth Härtig, Braunschweig, Germany Homepage

Your additional remarks

References

  1. Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed