Difference between revisions of "AraB"

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* '''Regulation:''' repressed by glucose ([[CcpA]])
 
* '''Regulation:''' repressed by glucose ([[CcpA]])
 +
** repressed in the absence of arabinose ([[AraR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
  
 
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression  
 
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression  
 +
** [[AraR]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10417639 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 11:44, 11 June 2009

  • Description: L-ribulokinase

Gene name araB
Synonyms
Essential no
Product L-ribulokinase
Function arabinose utilization
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 60 kDa, 5.233
Gene length, protein length 1680 bp, 560 aa
Immediate neighbours araD, araA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AraB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU28790

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L(or D)-ribulose = ADP + L(or D)-ribulose 5-phosphate (according to Swiss-Prot)
  • Protein family: ribulokinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA)
    • repressed in the absence of arabinose (AraR) PubMed
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Inácio JM, Costa C, de Sá-Nogueira I. (2003) Distinct molecular mechanisms involved in carbon catabolite repression of the arabinose regulon in Bacillus subtilis. Microbiology. Sep;149(Pt 9): 2345-55. PubMed
  2. Sa-Nogueira I.M.G., Nogueira T.V., Soares S., de Lencastre H. (1997) The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression. Microbiology. Mar;143(Pt 3): 957-69. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed