Difference between revisions of "Sandbox"
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− | * '''Description:''' | + | * '''Description:''' multidrug-efflux transporter <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
− | |'' | + | |''mdtP'' |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yusP '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || multidrug-efflux transporter |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || multidrug resistance |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || | + | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 57 kDa, 9.836 |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || | + | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1623 bp, 541 aa |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yusO]]'', ''[[yusQ]]'' |
|- | |- | ||
− | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS: | + | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15277]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' |
|- | |- | ||
− | |colspan="2" | '''Genetic context''' <br/> [[Image: | + | |colspan="2" | '''Genetic context''' <br/> [[Image:yusP_context.gif]] |
− | |||
|- | |- | ||
|} | |} | ||
Line 35: | Line 34: | ||
=== Basic information === | === Basic information === | ||
− | * '''Locus tag:''' | + | * '''Locus tag:''' |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
Line 41: | Line 40: | ||
=== Database entries === | === Database entries === | ||
− | * '''DBTBS entry:''' | + | * '''DBTBS entry:''' no entry |
− | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+ | + | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14028] |
=== Additional information=== | === Additional information=== | ||
Line 54: | Line 53: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
− | * '''Protein family:''' | + | * '''Protein family:''' EmrB family (according to Swiss-Prot) |
* '''Paralogous protein(s):''' | * '''Paralogous protein(s):''' | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
− | * '''Interactions:''' | + | * '''Interactions:''' |
− | * '''Localization:''' | + | * '''Localization:''' cell membrane (according to Swiss-Prot), membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed] |
=== Database entries === | === Database entries === | ||
Line 78: | Line 77: | ||
* '''Structure:''' | * '''Structure:''' | ||
− | * '''Swiss prot entry:''' | + | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O32182 O32182] |
− | * '''KEGG entry:''' | + | * '''KEGG entry:''' |
* '''E.C. number:''' | * '''E.C. number:''' | ||
Line 88: | Line 87: | ||
=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' ''[[ | + | * '''Operon:''' ''[[mdtR]]-[[mdtP]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19286808 PubMed] |
− | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' |
− | * '''Regulation:''' | + | * '''Regulation:''' |
− | * '''Regulatory mechanism:''' [[ | + | * '''Regulatory mechanism:''' repression by [[MdtR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19286808 PubMed] |
* '''Additional information:''' | * '''Additional information:''' | ||
Line 100: | Line 99: | ||
=Biological materials = | =Biological materials = | ||
− | * '''Mutant:''' | + | * '''Mutant:''' |
* '''Expression vector:''' | * '''Expression vector:''' | ||
Line 118: | Line 117: | ||
=References= | =References= | ||
− | <pubmed> | + | <pubmed>18763711 19286808, </pubmed> |
− | # | + | # Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing ''Bacillus subtilis'' by divergent approaches. Proteomics '''8:''' 4123-4136 [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed] |
+ | # Kim JY, Inaoka T, Hirooka K, Matsuoka H, Murata M, Ohki R, Adachi Y, Fujita Y, Ochi K (2009) Identification and characterization of a novel multidrug resistance operon ''mdtRP'' (''yusOP'') of ''Bacillus subtilis''. ''J. Bacteriol'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/19286808 PubMed] | ||
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] |
Revision as of 13:24, 8 June 2009
- Description: multidrug-efflux transporter
Gene name | mdtP |
Synonyms | yusP |
Essential | no |
Product | multidrug-efflux transporter |
Function | multidrug resistance |
MW, pI | 57 kDa, 9.836 |
Gene length, protein length | 1623 bp, 541 aa |
Immediate neighbours | yusO, yusQ |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context |
Contents
The gene
Basic information
- Locus tag:
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: EmrB family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot), membrane associated PubMed
Database entries
- Structure:
- Swiss prot entry: O32182
- KEGG entry:
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ji-Yun Kim, Takashi Inaoka, Kazutaka Hirooka, Hiroshi Matsuoka, Makiko Murata, Reiko Ohki, Yoshikazu Adachi, Yasutaro Fujita, Kozo Ochi
Identification and characterization of a novel multidrug resistance operon, mdtRP (yusOP), of Bacillus subtilis.
J Bacteriol: 2009, 191(10);3273-81
[PubMed:19286808]
[WorldCat.org]
[DOI]
(I p)
Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711]
[WorldCat.org]
[DOI]
(I p)
- Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
- Kim JY, Inaoka T, Hirooka K, Matsuoka H, Murata M, Ohki R, Adachi Y, Fujita Y, Ochi K (2009) Identification and characterization of a novel multidrug resistance operon mdtRP (yusOP) of Bacillus subtilis. J. Bacteriol volume: page-page. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed