Difference between revisions of "Sandbox"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
−
* '''Description:''' UDP glucose 4-epimerase <br/><br/>
+
* '''Description:''' glycine betaine-aldehyde dehydrogenase, glycine betaine synthesis <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''galE''
+
|''gbsA''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || UDP glucose 4-epimerase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || glycine betaine-aldehyde dehydrogenase
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || galactose utilization
+
|style="background:#ABCDEF;" align="center"|'''Function''' || osmoprotection
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 4.776  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 53 kDa, 4.713  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1017 bp, 339 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1470 bp, 490 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxkC]]'', ''[[yxkA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gbsB]]'', ''[[yuaC]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15912&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15084&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:galE_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:gbsA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 35: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU38860
+
* '''Locus tag:''' BSU31060
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/galE.html]
+
* '''DBTBS entry:''' no entry
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11837]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11940]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 52:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:''' UDP-glucose = UDP-galactose (according to Swiss-Prot)  
+
* '''Catalyzed reaction/ biological activity:''' Betaine aldehyde + NAD<sup>+</sup> + H<sub>2</sub>O = betaine + NADH (according to Swiss-Prot)  
  
−
* '''Protein family:''' sugar epimerase family (according to Swiss-Prot)
+
* '''Protein family:''' aldehyde dehydrogenase family (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 78: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
−
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P55180 P55180]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P71016 P71016]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU38860]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU31060]
  
−
* '''E.C. number:''' [http://www.expasy.org/enzyme/5.1.3.2 5.1.3.2]
+
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.2.1.8 1.2.1.8]
  
 
=== Additional information===
 
=== Additional information===
Line 113: Line 113:
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
[[Erhard Bremer]], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
  
 
=Your additional remarks=
 
=Your additional remarks=
Line 118: Line 120:
 
=References=
 
=References=
  
−
<pubmed>9555917, </pubmed>
+
<pubmed>9297465, </pubmed>
−
# Krispin O, Allmansberger R. (1998) The Bacillus subtilis galE gene is essential in the presence of glucose and galactose. '' J Bacteriol.'' '''Apr;180(8):''' 2265-70. [http://www.ncbi.nlm.nih.gov/sites/entrez/9555917 PubMed]
+
# Boch J, Nau-Wagner G, Kneip S, Bremer E. (1997) Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine. ''Arch Microbiol.'' '''Oct;168(4):''' 282-9. [http://www.ncbi.nlm.nih.gov/sites/entrez/9297465 PubMed]

Revision as of 13:18, 8 June 2009

  • Description: glycine betaine-aldehyde dehydrogenase, glycine betaine synthesis

Gene name gbsA
Synonyms
Essential no
Product glycine betaine-aldehyde dehydrogenase
Function osmoprotection
MW, pI 53 kDa, 4.713
Gene length, protein length 1470 bp, 490 aa
Immediate neighbours gbsB, yuaC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GbsA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU31060

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Betaine aldehyde + NAD+ + H2O = betaine + NADH (according to Swiss-Prot)
  • Protein family: aldehyde dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

Your additional remarks

References

J Boch, G Nau-Wagner, S Kneip, E Bremer
Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine.
Arch Microbiol: 1997, 168(4);282-9
[PubMed:9297465] [WorldCat.org] [DOI] (P p)

  1. Boch J, Nau-Wagner G, Kneip S, Bremer E. (1997) Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine. Arch Microbiol. Oct;168(4): 282-9. PubMed
Retrieved from "https://subtiwiki.uni-goettingen.de/wiki//index.php?title=Sandbox&oldid=88834"