Difference between revisions of "Sandbox"
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− | * '''Description:''' | + | * '''Description:''' 6S RNA<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
− | |'' | + | |''bsrA'' |
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || 6S RNA |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || antagonist of [[SigA]] |
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− | |style="background:#ABCDEF;" align="center"| ''' | + | |style="background:#ABCDEF;" align="center"| '''Gene length''' || 190 bp |
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− | |style="background:#ABCDEF;" align="center"| ''' | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrvM]]'', ''[[aspS]]'' |
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− | |style="background:# | + | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE.txt Gene sequence (+200bp) ]''' |
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− | + | |colspan="2" | '''Genetic context''' <br/> [[Image:BsrA_context.jpeg]] | |
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− | |colspan="2" | '''Genetic context''' <br/> [[Image: | ||
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|} | |} | ||
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<br/><br/> | <br/><br/> | ||
+ | <br/><br/><br/><br/><br/> | ||
=The gene= | =The gene= | ||
=== Basic information === | === Basic information === | ||
− | * '''Locus tag:''' | + | * '''Locus tag:''' |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
− | + | reduced growth rate [http://www.ncbi.nlm.nih.gov/sites/entrez/12177353 PubMed] | |
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=== Database entries === | === Database entries === | ||
− | * ''' | + | * '''DBTBS entry:''' no entry |
− | * ''' | + | * '''SubtiList entry:''' no entry |
+ | |||
+ | * '''Structure:''' | ||
− | + | === Additional information=== | |
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=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' | + | * '''Operon:''' ''bsrA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12177353 PubMed] |
− | * ''' | + | * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12177353 PubMed] |
− | * '''Regulation:''' | + | * '''Regulation:''' expressed in vegetative cells [http://www.ncbi.nlm.nih.gov/sites/entrez/12177353 PubMed] |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
− | * '''Additional information:''' | + | * '''Additional information:''' transcribed as 201 nt RNA, processed to mature 190 bp 6S RNA [http://www.ncbi.nlm.nih.gov/sites/entrez/12177353 PubMed] |
=Biological materials = | =Biological materials = | ||
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* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
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− | + | =Labs working on this gene/RNA= | |
− | + | Kouji Nakamura, Tsukuba, University, Japan | |
=Your additional remarks= | =Your additional remarks= | ||
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=References= | =References= | ||
− | <pubmed> | + | <pubmed>12177353 15811922, </pubmed> |
− | # | + | # Suzuma S, Asari S, Bunai K, Yoshino K, Ando Y, Kakeshita H, Fujita M, Nakamura K, Yamane K (2002) Identification and characterization of novel small RNAs in the ''aspS-yrvM'' intergenic region of the ''Bacillus subtilis'' genome.. ''Microbiology'' '''148:''' 2591-2598. [http://www.ncbi.nlm.nih.gov/sites/entrez/12177353 PubMed] |
+ | # Barrick JE, Sudarsan N, Weinberg Z, Ruzzo WL, Breaker RR(2005) 6S RNA is a widespread regulator of eubacterial RNA polymerase that resembles an open promoter.''RNA'' '''11:''' 774-784. [http://www.ncbi.nlm.nih.gov/sites/entrez/15811922 PubMed] | ||
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] |
Revision as of 13:10, 8 June 2009
- Description: 6S RNA
Gene name | bsrA |
Synonyms | |
Essential | no |
Product | 6S RNA |
Function | antagonist of SigA |
Gene length | 190 bp |
Immediate neighbours | yrvM, aspS |
Gene sequence (+200bp) | |
Genetic context |
Contents
The gene
Basic information
- Locus tag:
Phenotypes of a mutant
reduced growth rate PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: no entry
- Structure:
Additional information
Expression and regulation
- Operon: bsrA PubMed
- Regulation: expressed in vegetative cells PubMed
- Regulatory mechanism:
- Additional information: transcribed as 201 nt RNA, processed to mature 190 bp 6S RNA PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
Labs working on this gene/RNA
Kouji Nakamura, Tsukuba, University, Japan
Your additional remarks
References
Jeffrey E Barrick, Narasimhan Sudarsan, Zasha Weinberg, Walter L Ruzzo, Ronald R Breaker
6S RNA is a widespread regulator of eubacterial RNA polymerase that resembles an open promoter.
RNA: 2005, 11(5);774-84
[PubMed:15811922]
[WorldCat.org]
[DOI]
(P p)
Satoru Suzuma, Sayaka Asari, Keigo Bunai, Keiko Yoshino, Yoshinari Ando, Hiroshi Kakeshita, Masaya Fujita, Kouji Nakamura, Kunio Yamane
Identification and characterization of novel small RNAs in the aspS-yrvM intergenic region of the Bacillus subtilis genome.
Microbiology (Reading): 2002, 148(Pt 8);2591-2598
[PubMed:12177353]
[WorldCat.org]
[DOI]
(P p)
- Suzuma S, Asari S, Bunai K, Yoshino K, Ando Y, Kakeshita H, Fujita M, Nakamura K, Yamane K (2002) Identification and characterization of novel small RNAs in the aspS-yrvM intergenic region of the Bacillus subtilis genome.. Microbiology 148: 2591-2598. PubMed
- Barrick JE, Sudarsan N, Weinberg Z, Ruzzo WL, Breaker RR(2005) 6S RNA is a widespread regulator of eubacterial RNA polymerase that resembles an open promoter.RNA 11: 774-784. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed