Difference between revisions of "CsaA"
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | From unsuccessful trials to knock out the gene it was concluded that csaA apparently is essential. However, in a conditional mutant (Pspac-csaA) depletion of the gene product is not lethal. But secretion of some proteins including | + | From unsuccessful trials to knock out the gene it was concluded that ''csaA'' apparently is essential. However, in a conditional mutant (Pspac-''csaA''), depletion of the gene product is not lethal. But secretion of some proteins including [[SdpC]] was reported to be affected unter such conditions.[http://www.ncbi.nlm.nih.gov/pubmed/10816431 PubMed] |
=== Database entries === | === Database entries === | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' Secretion associated chaperone. Assumed functional analogue of E. coli SecB | + | * '''Catalyzed reaction/ biological activity:''' Secretion associated chaperone. Assumed functional analogue of ''E. coli'' SecB |
* '''Protein family:''' FIG1 subfamily (according to Swiss-Prot) | * '''Protein family:''' FIG1 subfamily (according to Swiss-Prot) | ||
Revision as of 17:08, 5 June 2009
- Description: molecular chaperone involved in protein secretion
| Gene name | csaA |
| Synonyms | |
| Essential | no |
| Product | molecular chaperone |
| Function | protein secretion |
| MW, pI | 11 kDa, 8.691 |
| Gene length, protein length | 330 bp, 110 aa |
| Immediate neighbours | bsrF, yobQ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU19040
Phenotypes of a mutant
From unsuccessful trials to knock out the gene it was concluded that csaA apparently is essential. However, in a conditional mutant (Pspac-csaA), depletion of the gene product is not lethal. But secretion of some proteins including SdpC was reported to be affected unter such conditions.PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Secretion associated chaperone. Assumed functional analogue of E. coli SecB
- Protein family: FIG1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: CsaA contains a tRNA binding domain.
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure: 2NZO
- Swiss prot entry: P37584
- KEGG entry: [2]
- E.C. number:
Additional information
The functional entity of CsaA is the homodimer.
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: Pspac-csaA PubMed
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
