Difference between revisions of "IleS"
| Line 37: | Line 37: | ||
=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU15430 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 84: | Line 84: | ||
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q45477 Q45477] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q45477 Q45477] | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15430] |
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.1.1.5 6.1.1.5] | * '''E.C. number:''' [http://www.expasy.org/enzyme/6.1.1.5 6.1.1.5] | ||
Revision as of 09:37, 3 June 2009
- Description: isoleucyl-tRNA synthetase
| Gene name | ileS |
| Synonyms | |
| Essential | yes PubMed |
| Product | isoleucyl-tRNA synthetase |
| Function | translation |
| MW, pI | 104 kDa, 5.191 |
| Gene length, protein length | 2763 bp, 921 aa |
| Immediate neighbours | divIVA, ylyA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU15430
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile) (according to Swiss-Prot)
- Protein family: IleS type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: Q45477
- KEGG entry: [2]
- E.C. number: 6.1.1.5
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Scott P Salowe, Judyann Wiltsie, Julio C Hawkins, Lisa M Sonatore
The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase.
J Biol Chem: 2009, 284(15);9656-62
[PubMed:19233850]
[WorldCat.org]
[DOI]
(P p)
Ulf Gerth, Holger Kock, Ilja Kusters, Stephan Michalik, Robert L Switzer, Michael Hecker
Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis.
J Bacteriol: 2008, 190(1);321-31
[PubMed:17981983]
[WorldCat.org]
[DOI]
(I p)
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
