Difference between revisions of "PrkC"

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(References)
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=References=
 
=References=
  
# Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model  bacterium ''Bacillus subtilis''. Mol. Cell. Proteomics 6: 697-707  [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed]
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<pubmed> 17218307, 12399479, 12406230, 19246764, 12842463 , 18984160 </pubmed>
# Gaidenko TA, Kim TJ, Price CW: (2002) The PrpC serine-threonine phosphatase and PrkC kinase have opposing physiological roles in stationary-phase Bacillus subtilis cells. J Bacteriol, 184:6109-6114. [http://www.ncbi.nlm.nih.gov/sites/entrez/12399479 PubMed]
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# Madec E, Laszkiewicz A, Iwanicki A, Obuchowski M, Séror S: (2002) Characterization of a membrane-linked Ser/Thr protein kinase in Bacillus subtilis, implicated in developmental processes. Mol Microbiol, 46:571-586. [http://www.ncbi.nlm.nih.gov/sites/entrez/12406230 PubMed]
 
# Madec E, Stensballe A, Kjellström S, Cladière L, Obuchowski M, Jensen ON, Séror S: (2003) Mass spectrometry and site-directed mutagenesis identify several autophosphorylated residues required for the activity of PrkC, a Ser/Thr kinase from Bacillus subtilis. J Mol Biol 330:459-472. [http://www.ncbi.nlm.nih.gov/sites/entrez/12842463 PubMed]
 
# Shah IM, Laaberki MH, Popham DL, Dworkin J: A eukaryotic-like Ser/Thr kinase signals bacteria to exit dormancy in response to peptidoglycan fragments. Cell 2008, 135:486-496. [http://www.ncbi.nlm.nih.gov/sites/entrez/18984160 PubMed]
 
# Absalon C, Obuchowski M, Madec E, Delattre D, Holland IB, Séror SJ (2009) CpgA, EF-Tu and the stressosome protein YezB are substrates of the Ser/Thr kinase/phosphatase couple, PrkC/PrpC, in ''Bacillus subtilis''. ''Microbiology'' '''155:''' 932-943. [http://www.ncbi.nlm.nih.gov/sites/entrez/19246764 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:28, 31 May 2009

  • Description: protein kinase C

Gene name prkC
Synonyms yloP
Essential no
Product protein kinase
Function germination in response to muropeptides
MW, pI 71 kDa, 4.833
Gene length, protein length 1944 bp, 648 aa
Immediate neighbours prpC, cpgA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PrkC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
  • Protein family: protein kinase domain (according to Swiss-Prot)
  • Paralogous protein(s):

Proteins phosphorylated by PrkC

CpgA, EF-Tu, YezB PubMed, EF-G PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Thr-290 PubMed
  • Cofactor(s):
  • Effectors of protein activity: activated by muropeptides PubMed
  • Interactions:
  • Localization: membrane (according to Swiss-Prot), membrane PubMed

Database entries

  • Structure:
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector: pGP832 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References


  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed