FtsH

• Description: ATP-dependent metalloprotease
  
  

Gene name	ftsH
Synonyms
Essential	no
Product	ATP-dependent metalloprotease
Function	cell division, sporulation initiation
MW, pI	70 kDa, 5.841
Gene length, protein length	1911 bp, 637 aa
Immediate neighbours	hprT, coaX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

strongly reduced sporulation frequency

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: degrades Spo0E, resulting in reduced sporulation frequency in a ftsH mutant

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot), membrane PubMed

Database entries

• Structure:

• Swiss prot entry: P37476

• KEGG entry: BSU00690

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation: induced by heat shock (class III)

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ai Thi Thuy Le, Wolfgang Schumann
The Spo0E phosphatase of Bacillus subtilis is a substrate of the FtsH metalloprotease.
Microbiology (Reading): 2009, 155(Pt 4);1122-1132
[PubMed:19332814] [WorldCat.org] [DOI] (P p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed