Difference between revisions of "LipA"
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' Triacylglycerol + H<sub>2</sub>O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for ''[[comEA]]'' transcription [http://www.ncbi.nlm.nih.gov/sites/entrez/19028902 PubMed] | + | * '''Catalyzed reaction/ biological activity:''' Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H<sub>2</sub>O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for ''[[comEA]]'' transcription [http://www.ncbi.nlm.nih.gov/sites/entrez/19028902 PubMed] |
* '''Protein family:''' Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot) | * '''Protein family:''' Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot) | ||
Revision as of 13:38, 24 May 2009
- Description: trigger enzyme: lipoic acid synthetase and regulator of comE operon expression
| Gene name | lipA |
| Synonyms | yutB |
| Essential | no |
| Product | lipoic acid synthetase |
| Function | lipid metabolism |
| MW, pI | 33 kDa, 8.251 |
| Gene length, protein length | 894 bp, 298 aa |
| Immediate neighbours | yutC, lytH |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
reduced transformation efficiency PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol + H2O = diacylglycerol + a carboxylate (according to Swiss-Prot) required for comEA transcription PubMed
- Protein family: Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: extracellular (signal peptide) PubMed
Database entries
- Structure:
- E.C. number: 2.8.1.8 3.1.1.3]
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
- Ogura, M. and T. Tanaka (2009) The Bacillus subtilis Late Competence Operon comE Is Transcriptionally Regulated by yutB and under Post-Transcription Initiation Control by comN (yrzD) J. Bacteriol. 191: 949-958. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
