Difference between revisions of "LexA"

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(Basic information/ Evolution)
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' Hydrolysis of Ala-|-Gly bond in repressor lexA (according to Swiss-Prot)  
+
* '''Catalyzed reaction/ biological activity:''' Represses ''[[dinA]], [[dinB]], [[dinC]], [[recA]]'' genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, [[RecA]] interacts with [[LexA]] causing an autocatalytic cleavage which disrupts the DNA-binding part of [[LexA]], leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)  
  
 
* '''Protein family:''' peptidase S24 family (according to Swiss-Prot)
 
* '''Protein family:''' peptidase S24 family (according to Swiss-Prot)

Revision as of 13:06, 24 May 2009

  • Description: transcriptional repressor of the SOS regulon

Gene name lexA
Synonyms dinR
Essential no
Product transcriptional repressor of the SOS regulon
Function regulation of DNA damage repair
MW, pI 22 kDa, 5.155
Gene length, protein length 615 bp, 205 aa
Immediate neighbours fosB, yneA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LexA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Represses dinA, dinB, dinC, recA genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)
  • Protein family: peptidase S24 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: autoregulation by LexA PubMed
  • Regulatory mechanism: autorepression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed