Difference between revisions of "DegQ"
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− | * '''Description:''' degradative | + | * '''Description:''' stimulates production of degradative enzymes and of extracellular poly-gamma-glutamate [http://www.ncbi.nlm.nih.gov/sites/entrez/16091050 PubMed], stimulates phosphorylation of [[DegU]] by [[DegS]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17850253 PubMed], gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) [http://www.ncbi.nlm.nih.gov/sites/entrez/16091050 PubMed]<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Gene name''' | + | |style="background:#ABCDEF;" align="center"|'''Gene name''' |
|''degQ'' | |''degQ'' | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
+ | |||
+ | loss of poly-gamma-glutamate production [http://www.ncbi.nlm.nih.gov/sites/entrez/16091050 PubMed], no synthesis of nonribosomal peptides iturin A and plipastatin [http://www.ncbi.nlm.nih.gov/sites/entrez/16251307 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/10471562 PubMed2], reduced synthesis of degradative enzymes [http://www.ncbi.nlm.nih.gov/sites/entrez/3098732 PubMed], increased genetic competence [http://www.ncbi.nlm.nih.gov/sites/entrez/3098732 PubMed] | ||
=== Database entries === | === Database entries === | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
− | * '''Interactions:''' | + | * '''Interactions:''' [[DegQ]]-[[DegS]] |
* '''Localization:''' | * '''Localization:''' | ||
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* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
− | * '''Regulation:''' | + | * '''Regulation:''' expression is induced by high cell density ([[ComA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1901055 PubMed],subject to carbon catabolite repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1901055 PubMed], controlled by [[DegU]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1901055 PubMed] |
− | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' [[ComA]]: transcription activation, [http://www.ncbi.nlm.nih.gov/sites/entrez/1901055 PubMed], [[DegU]]: transcription activation, [http://www.ncbi.nlm.nih.gov/sites/entrez/1901055 PubMed] |
− | * '''Additional information:''' | + | * '''Additional information:''' the gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) [http://www.ncbi.nlm.nih.gov/sites/entrez/16091050 PubMed] |
=Biological materials = | =Biological materials = |
Revision as of 08:58, 21 May 2009
- Description: stimulates production of degradative enzymes and of extracellular poly-gamma-glutamate PubMed, stimulates phosphorylation of DegU by DegS PubMed, gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed
Gene name | degQ |
Synonyms | sacQ |
Essential | no |
Product | pleiotropic regulator |
Function | regulation of exoenzyme synthesis |
MW, pI | 5 kDa, 6.536 |
Gene length, protein length | 138 bp, 46 aa |
Immediate neighbours | comQ, yuzC |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
loss of poly-gamma-glutamate production PubMed, no synthesis of nonribosomal peptides iturin A and plipastatin PubMed1 PubMed2, reduced synthesis of degradative enzymes PubMed, increased genetic competence PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- Swiss prot entry: Q99039
- KEGG entry: BSU31720
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation: expression is induced by high cell density (ComA) PubMed,subject to carbon catabolite repression PubMed, controlled by DegU PubMed
- Regulatory mechanism: ComA: transcription activation, PubMed, DegU: transcription activation, PubMed
- Additional information: the gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed