RapA

• Description: response regulator aspartate phosphatase
  
  

Gene name	rapA
Synonyms	gsiAA, spo0L
Essential	no
Product	response regulator aspartate phosphatase
Function	control of sporulation initiation
MW, pI	44 kDa, 4.848
Gene length, protein length	1134 bp, 378 aa
Immediate neighbours	yjoB, phrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: RapA-PhrA, RapA-Spo0F

• Localization:

Database entries

• Structure:

• Swiss prot entry: Q00828

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation: repressed by glucose (4.2-fold) (CcpA) PubMed, repressed by casamino acids PubMed

• Regulatory mechanism: CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
2. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed