Difference between revisions of "Sandbox"

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* '''Description:''' rRNA adenine dimethyltransferase <br/><br/>
+
* '''Description:''' unknown <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ksgA''
+
|''yabG''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || rRNA adenine dimethyltransferase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance to kasugamycin
+
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.712  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 9.143  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 876 bp, 292 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 870 bp, 290 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnmV]]'', ''[[yabG]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ksgA]]'', ''[[veg]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11818&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11819&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ksgA_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:yabG_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rnmV-ksgA.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yabG.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10105]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10106]
  
 
=== Additional information===
 
=== Additional information===
Line 72: Line 72:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:'''
+
* '''Localization:''' forespore outer membrane (according to Swiss-Prot),  spore coat [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
Line 78: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37468 P37468]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37548 P37548]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00420]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00430]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
 +
colocalizes in the spore coat with [[YeeK]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19060142 PubMed]
  
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[rnmV]]-[[ksgA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11233981 PubMed]  
+
* '''Operon:''' ''yabG'' [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigK]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
  
* '''Regulation:'''  
+
* '''Regulation:''' expression starts four hours after onset of sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
Line 118: Line 119:
 
=References=
 
=References=
  
# Condon et al. (2001) Identification of the gene encoding the 5S ribosomal RNA maturase in ''Bacillus subtilis'': mature 5S rRNA is dispensable for ribosome function. ''RNA'' '''7:''' 242-253. [http://www.ncbi.nlm.nih.gov/sites/entrez/11233981 PubMed]
+
# Takamatsu ''et al''. (2000) The ''Bacillus subtilis yabG'' gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition.''J. Bacteriol.'' '''182:''' 1883-1888. [http://www.ncbi.nlm.nih.gov/sites/entrez/10714992 PubMed]
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Revision as of 15:26, 4 May 2009

  • Description: unknown

Gene name yabG
Synonyms
Essential no
Product unknown
Function unknown
MW, pI 33 kDa, 9.143
Gene length, protein length 870 bp, 290 aa
Immediate neighbours ksgA, veg
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YabG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: forespore outer membrane (according to Swiss-Prot), spore coat PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

colocalizes in the spore coat with YeeK PubMed

Expression and regulation

  • Regulation: expression starts four hours after onset of sporulation PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Takamatsu et al. (2000) The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition.J. Bacteriol. 182: 1883-1888. PubMed