Difference between revisions of "Sandbox"

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* '''Description:''' RNA polymerase forespore-specific (early) [[sigma factor]] SigF <br/><br/>
+
* '''Description:''' Na+/H+ antiporter subunit, multiple resistance and pH homeostasis <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sigF''
+
|''mrpC''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spoIIAC ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yufV ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase forespore-specific (early) [[sigma factor]] SigF
+
|style="background:#ABCDEF;" align="center"| '''Product''' || Na+/H+ antiporter subunit
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || transcription of sporulation genes (late mother cell)
+
|style="background:#ABCDEF;" align="center"|'''Function''' || sodium export
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.078  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 6.475  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 765 bp, 255 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 339 bp, 113 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoVAA]]'', ''[[spoIIAB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mrpB]]'', ''[[mrpD]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14277&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15151&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:sigF_context.gif]]
+
|-
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:mrpC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 38: Line 40:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dacF-spoIIAAB-sigF.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mrpABCDEFG.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10298]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12357]
  
 
=== Additional information===
 
=== Additional information===
Line 57: Line 61:
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
 
=== Genes controlled by SigF ===
 
 
''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'', ''[[sigG]]'','' [[pbpF]]'', ''[[yphA]]-[[seaA]]''
 
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 84: Line 84:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23450]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU31620]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 92: Line 92:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]''
+
* '''Operon:'''  
''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]''
 
  
* '''[[Sigma factor]]:''' ''[[dacF]]'': [[SigF]], [[SigG]]
+
* '''[[Sigma factor]]:'''  
''[[spoIIA]]'': [[SigH]]
 
  
* '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] ,  ''[[dacF]]'': expressed during sporulation, ''[[spoIIAA]]'': expressed early during sporulation
+
* '''Regulation:'''  
  
* '''Regulatory mechanism:''' ''[[spoIIAA]]'': activated by [[Spo0A]] and repressed by [[SinR]]
+
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
Line 124: Line 122:
 
=References=
 
=References=
  
# M&#228;der et al. (2002) Transcriptome and Proteome Analysis of ''Bacillus subtilis'' Gene Expression Modulated by Amino Acid Availability. ''J. Bacteriol'' '''184:''' 1844288-4295 [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 08:23, 27 April 2009

  • Description: Na+/H+ antiporter subunit, multiple resistance and pH homeostasis

Gene name mrpC
Synonyms yufV
Essential yes PubMed
Product Na+/H+ antiporter subunit
Function sodium export
MW, pI 12 kDa, 6.475
Gene length, protein length 339 bp, 113 aa
Immediate neighbours mrpB, mrpD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MrpC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed