Difference between revisions of "Sandbox"

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* '''Description:''' transcriptional repressor of the gluconate operon <br/><br/>
+
#REDIRECT [[bacA]]
 
 
{| align="right" border="1" cellpadding="2"
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|''gntR''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor of the gluconate operon
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of gluconate utilization
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 5.26 
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 729 bp, 243 aa
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxaA]]'', ''[[gntK]]''
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB16042&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:gntR_context.gif]]
 
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|}
 
 
 
__TOC__
 
 
 
<br/><br/>
 
 
 
=The gene=
 
 
 
=== Basic information ===
 
 
 
* '''Coordinates:'''
 
 
 
===Phenotypes of a mutant ===
 
 
 
=== Database entries ===
 
 
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gntRKPZ.html]
 
 
 
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10648]
 
 
 
=== Additional information===
 
 
 
 
 
=The protein=
 
 
 
=== Basic information/ Evolution ===
 
 
 
* '''Catalyzed reaction/ biological activity:'''
 
 
 
* '''Protein family:'''
 
 
 
* '''Paralogous protein(s):'''
 
 
 
=== Extended information on the protein ===
 
 
 
* '''Kinetic information:'''
 
 
 
* '''Domains:'''
 
 
 
* '''Modification:'''
 
 
 
* '''Cofactor(s):'''
 
 
 
* '''Effectors of protein activity:'''
 
 
 
* '''Interactions:'''
 
 
 
* '''Localization:'''
 
 
 
=== Database entries ===
 
 
 
* '''Structure:'''
 
 
 
* '''Swiss prot entry:'''
 
 
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU40050]
 
 
 
* '''E.C. number:'''
 
 
 
=== Additional information===
 
 
 
=Expression and regulation=
 
 
 
* '''Operon:''' ''[[gntR]]-[[gntK]]-[[gntP]]-[[gntZ]]''
 
 
 
* '''[[Sigma factor]]:''' [[SigA]]
 
 
 
* '''Regulation:'''
 
 
 
* '''Regulatory mechanism:'''
 
 
 
* '''Additional information:'''
 
 
 
=Biological materials =
 
 
 
* '''Mutant:'''
 
 
 
* '''Expression vector:'''
 
       
 
* '''lacZ fusion:'''
 
 
 
* '''GFP fusion:'''
 
 
 
* '''two-hybrid system:'''
 
 
 
* '''Antibody:'''
 
 
 
=Labs working on this gene/protein=
 
 
 
=Your additional remarks=
 
 
 
=References=
 
 
 
# Yoshida K, Miwa Y, Ohmori H, Fujita Y. (1995) Analysis of an insertional operator mutation (gntOi) that affects the expression level of the Bacillus subtilis gnt operon, and characterization of gntOi suppressor mutations. ''Mol Gen Genet.'' '''Sep 20;248(5):''' 583-91. [http://www.ncbi.nlm.nih.gov/sites/entrez/7476858 PubMed]
 
# Fujita Y, Fujita T, Miwa Y, Nihashi J, Aratani Y. (1986) Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis. ''J Biol Chem.'' '''Oct 15;261(29):''' 13744-53. [http://www.ncbi.nlm.nih.gov/sites/entrez/3020045 PubMed]
 
# Reizer A., Deutscher J., Saier M.H. Jr., Reizer J. (1991) Analysis of the gluconate (gnt) operon of Bacillus subtilis. ''Mol. Microbiol.'' '''5:''' 1081-1089. [http://www.ncbi.nlm.nih.gov/sites/entrez/1659648 PubMed]
 

Revision as of 12:09, 25 April 2009

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