Difference between revisions of "Sandbox"

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* '''Description:''' unknown <br/><br/>
+
* '''Description:''' replicative DNA helicase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
||-
 
||-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yycA''
+
|''dnaC''
 
||-
 
||-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
||-
 
||-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
||-
 
||-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || replicative DNA helicase
 
||-
 
||-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA replication
 
||-
 
||-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 73 kDa, 9.678  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 4.548  
 
||-
 
||-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2055 bp, 685 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1362 bp, 454 aa  
 
||-
 
||-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yycB]]'', ''[[rplI]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yycE]]'', ''[[yycD]]''
 
||-
 
||-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
||-
 
||-
 
|-||-
 
|-||-
|colspan="2" | '''Genetic context''' <br/> [[Image:yycA_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:dnaC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
||-
 
||-
Line 30: Line 30:
 
__TOC__
 
__TOC__
  
<br/><br/>
+
<br/><br/><br/><br/>
  
 
=The gene=
 
=The gene=
Line 39: Line 39:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' no entry
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dnaC.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10008]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10004]
  
 
=== Additional information===
 
=== Additional information===
Line 57: Line 59:
 
* '''Protein family:'''
 
* '''Protein family:'''
  
* '''Paralogous protein(s):''' [[YkcB]]
+
* '''Paralogous protein(s):'''
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 71: Line 73:
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:'''
+
* '''Interactions:''' [[DnaI]]|-[[DnaC]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed]
  
* '''Localization:'''
+
* '''Localization:''' Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget|-bin/www_bget?bsu+BSU40490]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget|-bin/www_bget?bsu+BSU40440]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 91: Line 93:
 
* '''Operon:'''  
 
* '''Operon:'''  
  
* '''[[[[Sigma factor]]]]:'''  
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 119: Line 121:
 
=References=
 
=References=
  
 +
# Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in ''Bacillus subtilis'': identification of new proteins at the DNA replication factory ''Proteomics'' '''6:''' 2135|-2146. [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
 +
# Imai et al. (2000) Subcellular localization of Dna|-initiation proteins of ''Bacillus subtilis'': evidence that chromosome replication begins at either edge of the nucleoids. ''Mol. Microbiol.'' '''36:''' 1037|-1048. [http://www.ncbi.nlm.nih.gov/sites/entrez/10844689 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page|-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page|-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:27, 15 April 2009

  • Description: replicative DNA helicase

- Gene name dnaC - Synonyms - Essential yes PubMed - Product replicative DNA helicase - Function DNA replication - MW, pI 50 kDa, 4.548 - Gene length, protein length 1362 bp, 454 aa - Immediate neighbours yycE, yycD - Hier soll was neues rein -
Genetic context
DnaC context.gif
This image was kindly provided by SubtiList
-





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: Cytoplasm (Homogeneous) PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two|-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135|-2146. PubMed
  2. Imai et al. (2000) Subcellular localization of Dna|-initiation proteins of Bacillus subtilis: evidence that chromosome replication begins at either edge of the nucleoids. Mol. Microbiol. 36: 1037|-1048. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page|-page. PubMed