Difference between revisions of "PAC7"
| Line 1: | Line 1: | ||
[[File:PAC7.jpeg|250px|thumb|right|'''pAC7: click to enlarge''']] | [[File:PAC7.jpeg|250px|thumb|right|'''pAC7: click to enlarge''']] | ||
| + | [[File:PAC7_cloning_region.jpeg|250px|thumb|right|'''pAC7 cloning region: click to enlarge''']] | ||
pAC7 | pAC7 | ||
| Line 14: | Line 15: | ||
similar vectors: [[pAC5]], [[pAC6]] | similar vectors: [[pAC5]], [[pAC6]] | ||
| + | |||
| + | sequencing primer: | ||
| + | *'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’ | ||
| + | *'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘ | ||
| + | *'''Kancheckrev:''' 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘ | ||
| + | |||
Weinrauch et al. (1991) J Bacteriol. 173:5685-5693. [http://www.ncbi.nlm.nih.gov/sites/entrez/1715859 PubMed] | Weinrauch et al. (1991) J Bacteriol. 173:5685-5693. [http://www.ncbi.nlm.nih.gov/sites/entrez/1715859 PubMed] | ||
Revision as of 12:03, 18 March 2009
pAC7
vector for the construction of translational lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: kanamycin resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Kancheckrev: 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘
Weinrauch et al. (1991) J Bacteriol. 173:5685-5693. PubMed
