Difference between revisions of "PETM-11"
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* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease | * vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease | ||
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* in ''E. coli'': ampicillin resistance | * in ''E. coli'': ampicillin resistance | ||
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*sequencing primer: | *sequencing primer: | ||
*'''CZ103'''</font>: 5’-GAGGATCGAGATCTCGATC-3‘ | *'''CZ103'''</font>: 5’-GAGGATCGAGATCTCGATC-3‘ | ||
*'''CZ104''': 5’-CTAGTTATTGCTCAGCGGTG-3‘ | *'''CZ104''': 5’-CTAGTTATTGCTCAGCGGTG-3‘ | ||
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<pubmed> 16351710 </pubmed> | <pubmed> 16351710 </pubmed> | ||
== Return to [[Plasmids]] == | == Return to [[Plasmids]] == | ||
Revision as of 09:54, 19 November 2015
- vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease
- host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
- in E. coli: ampicillin resistance
- from EMBL
- sequencing primer:
- CZ103</font>: 5’-GAGGATCGAGATCTCGATC-3‘
- CZ104: 5’-CTAGTTATTGCTCAGCGGTG-3‘
Annett Dümmler, Ann-Marie Lawrence, Ario de Marco
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors.
Microb Cell Fact: 2005, 4;34
[PubMed:16351710]
[WorldCat.org]
[DOI]
(I e)
