RadA

• Description: 6-O-methylguanine-DNA methyltransferase, negative effector of DisA activity, suppressor of recU and recB mutations, participates in the stabilization and or processing of holliday junction intermediates
  
  

Gene name	radA
Synonyms	sms, yacJ
Essential	no
Product	6-O-methylguanine-DNA methyltransferase
Function	homologous recombination , control of c-di-AMP formation
Gene expression levels in SubtiExpress: radA
Interactions involving this protein in SubtInteract: RadA
Metabolic function and regulation of this protein in SubtiPathways: radA
MW, pI	49 kDa, 8.128
Gene length, protein length	1374 bp, 458 aa
Immediate neighbours	clpC, disA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides, DNA repair/ recombination, general stress proteins (controlled by SigB), heat shock proteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon

The gene

Basic information

• Locus tag: BSU00870

Database entries

• BsubCyc: BSU00870

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RadA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RadA-DisA PubMed

• Localization:

Database entries

• BsubCyc: BSU00870

• Structure:

• UniProt: P37572

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctsR-mcsA-mcsB-clpC-radA-disA PubMed

• Expression browser: radA PubMed

• Sigma factor: SigA PubMed, SigB PubMed1 PubMed2

• Regulation: induced by stress (SigB) PubMed, induced by heat (CtsR) PubMed

• Regulatory mechanism: CtsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • BKG1 (radA::spc), available in Jörg Stülke's lab
  • BKG2 (radA-disA::spc), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: