KtrC

• Description: low affinity potassium transporter KtrC-KtrD, peripheric membrane component
  
  

Gene name	ktrC
Synonyms	ylxV, yzaC, ykqB
Essential	no
Product	low affinity potassium transporter KtrC-KtrD, peripheric membrane component (proton symport)
Function	potassium uptake
Gene expression levels in SubtiExpress: ktrC
Interactions involving this protein in SubtInteract: KtrC
Metabolic function and regulation of this protein in SubtiPathways: Metal ion homeostasis, ktrC
MW, pI	24 kDa, 5.63
Gene length, protein length	663 bp, 221 aa
Immediate neighbours	ykqA, adeC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), coping with hyper-osmotic stress, membrane proteins

The gene

Basic information

• Locus tag: BSU14510

Database entries

• BsubCyc: BSU14510

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): KtrA

Extended information on the protein

• Kinetic information:

• Domains:
  • contains a RCK_N domain at the N-terminus (according to UniProt, [2])
  • contains a c-di-AMP-binding RCK_C domain at the C-terminus (according to UniProt, [3]) PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:
  • the protein binds c-di-AMP PubMed

• Interactions:
  • KtrC-KtrD PubMed

• Localization: peripheral membrane protein PubMed

Database entries

• BsubCyc: BSU14510

• Structure:

• UniProt: P39760

• KEGG entry: [4]

• E.C. number:

Expression and regulation

• Operon: ktrC PubMed

• Expression browser: ktrC PubMed

• Sigma factor:

• Regulation: constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 576 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 453 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 601 PubMed

Biological materials

• Mutant:
  • GHB6 (ktrC::spec), PubMed (available in Erhard Bremer's and Jörg Stülke's) labs, available at BGSC as 1A955
  • GP2079 (ktrC::tet), available in Jörg Stülke's lab
  • GP2083 (D(ktrA-ktrB)::aphA3 DktrC::tet), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

References

Reviews

Fabian M Commichau, Achim Dickmanns, Jan Gundlach, Ralf Ficner, Jörg Stülke
A jack of all trades: the multiple roles of the unique essential second messenger cyclic di-AMP.
Mol Microbiol: 2015, 97(2);189-204
[PubMed:25869574] [WorldCat.org] [DOI] (I p)

Original publications

Rebecca M Corrigan, Ivan Campeotto, Tharshika Jeganathan, Kevin G Roelofs, Vincent T Lee, Angelika Gründling
Systematic identification of conserved bacterial c-di-AMP receptor proteins.
Proc Natl Acad Sci U S A: 2013, 110(22);9084-9
[PubMed:23671116] [WorldCat.org] [DOI] (I p)

Gudrun Holtmann, Evert P Bakker, Nobuyuki Uozumi, Erhard Bremer
KtrAB and KtrCD: two K+ uptake systems in Bacillus subtilis and their role in adaptation to hypertonicity.
J Bacteriol: 2003, 185(4);1289-98
[PubMed:12562800] [WorldCat.org] [DOI] (P p)